原裝進(jìn)口細(xì)胞服務(wù)
我司經(jīng)過(guò)近年的發(fā)展,現(xiàn)與國(guó)外各大細(xì)胞及菌株機(jī)構(gòu)及廠家,建立了良好的合作關(guān)系。其中不乏ATCC、 Coriell 、DSMZ、JCRB、RIKEN,ECACC、BCRJ、Evercyte、Karafast、CLS、Sigma 等知名機(jī)構(gòu)和單位。
優(yōu)勢(shì):
● 質(zhì)量保障--國(guó)際運(yùn)輸全程液氮,國(guó)內(nèi)運(yùn)輸全程干冰。售后更有保證
● 貨期更優(yōu)--接到老師訂單確認(rèn)后,即刻安排專業(yè)人員準(zhǔn)備材料,向國(guó)外機(jī)構(gòu)訂購(gòu)
● 訂購(gòu)便捷--老師只需,提供簡(jiǎn)單研究方向等簡(jiǎn)要信息,其他文件由我司協(xié)助完成,大大節(jié)約老師時(shí)間與精力
我們致力于為客戶提供更高水平的專業(yè)細(xì)胞訂購(gòu)和技術(shù)支持。
德國(guó)DSMZ保藏中心成立于1969年,是德國(guó)的國(guó)家菌種保藏中心。該中心一直致力于細(xì)菌、真菌、質(zhì)粒、抗菌素、人體和動(dòng)物細(xì)胞、植物病毒等的分類、鑒定和保藏工作。
作為世界上*的保藏中心之一,DSMZ目前收藏超過(guò)70,000種產(chǎn)品,包括約31,000種不同的細(xì)菌和6,500種真菌菌株,830種人和動(dòng)物細(xì)胞系,1,500種植物病毒和抗血清,2,000種質(zhì)粒和噬菌體以及13,000種不同類型的細(xì)菌基因組DNA。
目前,DSMZ細(xì)胞系集合包含從多種組織以及許多雜交瘤中分離出來(lái)的840多種靈長(zhǎng)類,嚙齒動(dòng)物,兩棲動(dòng)物,魚類和昆蟲來(lái)源的永生細(xì)胞培養(yǎng)物
The DSMZcell lines collection currently comprises more than 840 different immortalizedcell cultures of primate, rodent, amphibian, fish, and insect origin isolatedfrom numerous tissues as well as a number of hybridomas
我們優(yōu)勢(shì)原裝進(jìn)口德國(guó)DSMZ的細(xì)胞及配套培養(yǎng)試劑,價(jià)格優(yōu)惠,貨期6-8周!
低溫產(chǎn)品提供全程干冰運(yùn)輸服務(wù),特殊細(xì)胞產(chǎn)品全程液氮運(yùn)輸,質(zhì)量和售后有保障!
PLB-985細(xì)胞產(chǎn)品信息:
ACC 139
Cell line: PLB-985
DSMZ no.: ACC 139
Species: human (Homo sapiens)
Cell type: acute myeloid leukemia (derivative of HL-60)
Origin: allegedly "established from the peripheral blood of a 38-year-oldwoman with acute myeloid leukemia (AML FAB M4) in relapse in 1985"; DNAfingerprinting showed unequivocally that PLB-985 is a subclone of cell lineHL-60
Reference(s): 14491 , 15024
Biosafety level: 1
Permissions and restrictions: A
DSMZ Cell Culture Data:
Morphology: single, round cells in suspension;
Medium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: seed out at ca. 1 x 106 cells/ml; maintain at ca.0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimumdensity at 0.2 x 106cells/ml
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 30 hours
Harvest: maximal density at about 1.5 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -;
Fingerprint: same DNA profile as HL-60 using multiplex PCR of minisatellitemarkers
Species: confirmed originally as human with IEF of AST, MDH and later by STRfingerprinting
Cytogenetics: human near diploid karyotype with 4% polyploidy -46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8-, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 400,- € | |
Growing culture | 800,- € | |
DNA isolated from cell line (25 μg) | 500,- € | |
DNA isolated from cell line (5 μg) | 120,- € |
我司現(xiàn)已保存有上百株國(guó)外機(jī)構(gòu)原裝細(xì)胞,同時(shí),我司也可提供部分穩(wěn)轉(zhuǎn)細(xì)胞系,歡迎老師隨時(shí)咨詢。
聯(lián)系人:邢先生 13260236521(同微信)
馬先生 18612979069(同微信)
或直接聯(lián)系我們,由總機(jī)為您服務(wù) 010-56146401
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